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Unit 7 · Topic 7.6

7.6 Evidence of Evolution

Evidence from many fields shows that life has changed over time and that species share common ancestors. Dated fossils, shared anatomy including vestigial structures, the geographic distribution of species, and similarities in DNA and protein sequences all point to the same branching history.

Key terms

  • fossil record
  • radiometric dating
  • homologous structure
  • vestigial structure
  • molecular evidence

Many independent lines of evidence

Evolution is supported by data from geography, geology, physics (radioactive decay), biochemistry and mathematics. What makes the case strong is that these independent kinds of evidence agree with each other. A family tree built from fossils matches one built from anatomy, which matches one built from DNA.

Fossils and how they're dated

Fossils are preserved remains or traces of organisms. They show that species living in the past were different from today's, and they include transitional forms. Tiktaalik, about 375 million years old, had features of both fish and early four-limbed animals, and fossil early whales had hind legs.

Scientists date fossils in several ways:

  • Age of the rock layers: sedimentary rock forms in layers, with younger layers on top of older ones. A fossil's layer gives its relative age.
  • Radiometric dating: radioactive isotopes decay at a steady rate, measured as a half-life (the time for half of the isotope to decay). Carbon-14 has a half-life of about 5,730 years and works for remains up to roughly 50,000 years old. Older rocks are dated with isotopes that have much longer half-lives, often in volcanic layers above and below a fossil.
  • Geographic data: where fossils are found, including on continents that have since drifted apart, helps place them in time and history.

Anatomy: homologous and vestigial structures

Homologous structures share the same underlying structure because they were inherited from a common ancestor, even if they now do different jobs. The forelimbs of humans, cats, whales and bats all have the same set of bones (one upper arm bone, two forearm bones, wrist bones and finger bones), used for grasping, walking, swimming and flying.

Vestigial structures are reduced remnants of structures that were functional in ancestors. Whales have small pelvic bones not attached to legs, some snakes have tiny hip and leg bones, and humans have a tailbone. These make sense only as inherited leftovers.

Be careful not to confuse these with analogous structures, like the wings of birds and insects, which do similar jobs but evolved independently (convergent evolution, 7.10). Analogous structures don't show common ancestry.

Molecules and biogeography

Comparing DNA nucleotide sequences or protein amino acid sequences gives some of the strongest evidence. Mutations accumulate over time, so species that split from a common ancestor more recently have fewer differences. Humans and chimpanzees, for example, have nearly identical sequences for many proteins.

Biogeography, the distribution of species across the planet, also fits. Species on islands usually resemble species on the nearest mainland (Galápagos species resemble South American ones), and Australia's many marsupials reflect its long isolation.

Worked examples

Try each one yourself first, then open the solution.

  1. Example 1

    Radiometric dating with half-lives

    A bone sample has 12.5% of the carbon-14 that a living organism has. The half-life of carbon-14 is about 5,730 years. Estimate the age of the bone.

    Show the solution
    1. Step 1: Each half-life cuts the remaining carbon-14 in half: 100% → 50% → 25% → 12.5%.
    2. Step 2: Reaching 12.5% takes 3 half-lives (½ × ½ × ½ = ⅛ = 12.5%).
    3. Step 3: Age ≈ 3 × 5,730 = 17,190 years.

    Answer: About 17,190 years old (roughly 17,000 years).

  2. Example 2

    Ranking relatedness from protein data

    The number of amino acid differences in one protein between species W and three other species is: species X, 2; species Y, 15; species Z, 31. Which species is most closely related to W, and what is the reasoning?

    Show the solution
    1. Step 1: Differences in a protein's amino acid sequence build up over time as mutations accumulate after two lineages split.
    2. Step 2: Fewer differences means less time since the lineages shared a common ancestor.
    3. Step 3: X has only 2 differences, Y has 15 and Z has 31.
    4. Step 4: So X shares the most recent common ancestor with W, then Y, then Z.

    Answer: Species X is most closely related to W (fewest differences, so the most recent common ancestor); Z is the most distantly related.

Common mistakes

  • Calling analogous structures homologous. Similar function from independent evolution (bird and insect wings) isn't evidence of common ancestry.
  • Saying carbon-14 can date dinosaur fossils. It's useful only up to about 50,000 years; much older fossils are dated with longer-lived isotopes in surrounding rock.
  • Thinking vestigial structures must be completely useless. They're reduced compared with their ancestral function; some still have minor uses.
  • Assuming fewer DNA differences means one species evolved from the other. It means they share a more recent common ancestor.

On the exam

  • Expect a data table of sequence differences or a diagram of bones and be asked what it shows about relatedness. State the claim and explain why the data support it.
  • If asked to describe evidence for evolution, give specific types (fossil dating, homologous or vestigial structures, molecular comparisons) rather than general statements.

Connected topics

Videos

  • 7.6 Evidence of Evolution - AP Biology (Updated 2025-2026)

    Gabe Poser - PoseKnows BiologyWatch on YouTube (opens in a new tab)

  • Evidence for evolution | Common ancestry and phylogeny | High school biology | Khan Academy

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  • What is the Evidence for Evolution?

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  • Evidence for evolution, Expertly Explained for AP Bio!

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Check yourself

4 questions on 7.6 Evidence of Evolution. Pick an answer to see if you got it, and why.

Question 1 of 4Calculator allowed

Carbon-14 has a half-life of about 5,730 years. A piece of bone contains 25 percent of the carbon-14 it had when the animal died. About how old is the bone?

Question 2 of 4

Whales have no hind legs, but many species have small pelvic bones embedded in their body wall. These bones have the same arrangement as the hip bones of land mammals. Which conclusion is best supported?

Question 3 of 4

A fossil is found in an undisturbed sedimentary rock layer. The layer lies directly above a volcanic ash layer dated by radioactive decay to 52 million years ago and directly below an ash layer dated to 48 million years ago. What can be concluded about the fossil's age?

Question 4 of 4

Species on a group of volcanic islands far off the coast of South America are most similar to species on the nearby South American mainland, even though the island climate is more like that of islands off Africa. Which of the following best explains this pattern?

0 of 4 answered